The PrimBio Microsatellite Instability (MSI) panel uses Ion Ampliseq technology to amplify 76 loci that are associated with MSI. These markers include the 5 original markers laid out by the Bethesda Panel for Colorectal Cancer and 71 other regions that are highly susceptible to insertions or deletions of nucleotide repeats. Due to the large number of microsatellite regions analyzed, this method of NGS analysis of MSI has been developed without matched normal tissue. Instead, the comparison of these microsatellite sequences to a reference human genome was able to provide the level of sensitivity achieved by the standard method. Thus, this MSI NGS panel makes it feasible to determine MSI status in patients lacking normal tissue.