PrimBio offers RNA-Seq services for a wide variety of species from mammalian to bacterial. Our goal is to assist researchers and clinicians in identifying significant DEGs, alternatively spliced transcripts, or low-abundance transcripts using our thorough RNA-seq workflow; which allows us to provide high quality reads with reproducible results. We can provide up to 120 million reads per sample using the Ion torrent 550 chip and the Ion S5 sequencer. In addition, our turnaround times are very competitive from library preparation to data analysis. All RNA-Seq services include total RNA Quality Control with Bioanalyzer, library preparation, sequencing, alignment and standard bioinformatics with the option of advanced bioinformatic analyses.
We offer three customized solutions for our RNAseq services:
1) Whole Transcriptome or mRNA-Seq- This service is for researchers or clinicians who want to analyze either the entire transcriptome or only the mRNA transcripts of a certain species. For this service we offer library preparation from rRNA-depleted total RNA samples for whole transcriptome, or mRNA enriched samples for mRNAseq. We prepare cDNA libraries using Ambion technology which includes attachment of adaptors and barcodes for multiplexing of samples and accurate identification of expression levels of transcripts.
2) Ampliseq Transcriptome- This service uses ampliseq technology to amplify targeted regions of the transcriptome, which covers approximately 20,000 RefSeq genes. Ampliseq transcriptome does not cover small RNAs, lnc RNAs or UTRs.
3) Small RNA-Seq- Small RNAs are non-coding RNA molecules (50-250 nucleotides long) that play an important role in gene silencing, post-transcriptional gene regulation, cell proliferation, differentiation and cell cycle progression. We use a magnetic bead procedure to enrich the small RNA, and the Ion Total RNA-Seq Kit V2 for library preparation. Depending on your request, we can sequence the small RNA library on the Ion S5 sequencer or Illumina NextSeq200 sequencer. We also provide small RNA-Seq bioinformatic analysis using StrandNGS software.
We offer three customized solutions for our RNAseq services:
1) Whole Transcriptome or mRNA-Seq- This service is for researchers or clinicians who want to analyze either the entire transcriptome or only the mRNA transcripts of a certain species. For this service we offer library preparation from rRNA-depleted total RNA samples for whole transcriptome, or mRNA enriched samples for mRNAseq. We prepare cDNA libraries using Ambion technology which includes attachment of adaptors and barcodes for multiplexing of samples and accurate identification of expression levels of transcripts.
2) Ampliseq Transcriptome- This service uses ampliseq technology to amplify targeted regions of the transcriptome, which covers approximately 20,000 RefSeq genes. Ampliseq transcriptome does not cover small RNAs, lnc RNAs or UTRs.
3) Small RNA-Seq- Small RNAs are non-coding RNA molecules (50-250 nucleotides long) that play an important role in gene silencing, post-transcriptional gene regulation, cell proliferation, differentiation and cell cycle progression. We use a magnetic bead procedure to enrich the small RNA, and the Ion Total RNA-Seq Kit V2 for library preparation. Depending on your request, we can sequence the small RNA library on the Ion S5 sequencer or Illumina NextSeq200 sequencer. We also provide small RNA-Seq bioinformatic analysis using StrandNGS software.
